Jekaterina Erenpreisa

Jekaterina ERENPREISA

Dr. habil. med. Jekaterina ERENPREISA
Latvian Biomedical Research and Study Centre

Ratsupites iela 1,
Riga, LV-1067
Latvia

Phone: +371 67808220
Fax: +371 67442407
e-mail: [email protected]

Born: February 13, 1945, Dnepropetrovsk, Ukraine

Interests:

Cell Nucleus Structure

Tumour Cell Biology

Chaotic regulations in Biological Systems

Brief Description of Main Results

1.    Basic research and development of new methods (approx. 16) in light and EM cytochemistry of cell nucleus.
2.    Studies of cell nucleus revealed co-operative character of levels of the chromatin organisation. In addition, discovered (1) Fractality of the rosette-like high level chromatin packaging (1989-1993); (2) Oscillatory character of the nuclear response to damage stress (1990-1993).
3.    Staining properties of tumour cell chromatin were found associated with superhelicity of DNA.
4.    Transdifferentiation in development and induction of differentiation in tumours were shown to involve asymmetric cell divisions and apoptosis.
5.    Aberrant cell cycle and cell death were found to have common features in embryonic development and tumours.
6.    Survival of the DNA damage-resistant tumours is likely due to the activation of the meiosis-like program in endopolyploid cells (1998- ).

Languages: English, Latvian, Russian (French, German)

Education

Medical Academy of Latvia (Faculty of General Medicine), 1968

Postgraduate course (Experimental Oncology), Latvian Institute of Experimental and Clinical Medicine, 1971

Dr. med. (Candidate of Science in former USSR, PhD in some Western countries), Latvian Institute of Experimental and Clinical Medicine, 1971

Dr.habil. med. (Doctor of Science in former USSR), Institute of Cancerogenesis Problems, Kiev, Ukraine, 1991.

Abroad:

Southampton University Course on Confocal Microscopy, England, 1997

EMBO Special Course on Digital Image Microscopy, Delft, The Netherlands, 1998.

Experience

Latvian Institute of Experimental and Clinical Medicine:

Junior Researcher, 1971-1981

Senior Researcher, 1981-1993.

A.Kirchenstein Institute of Microbiology and Virology, Riga:

Leading Researcher, 1993-1996

Head of the Laboratory of Tumour Cell Biology, 1997-2000

Biomedical Research and Study Centre, Riga

Leading Researcher, 2000 -

Honours and Awards

Corresponding Member, Latvian Academy of Sciences, 1999

Full Member, Latvian Academy of Sciences, 2003

Professional Activities and Memberships

Member of the Board, Latvian Morphologists Society, 1996 -

Member, Habilitation and Promotion Council (in Oncology), Institute of Experimental and Clinical Medicine, 1993

Member, New-York Academy of Sciences, 1996

Officer, International Union Against Cancer, 1996

Member, European Society of Analytical Cell Pathology, 1996

Member, European Tissue Culture Society, 1995

Member, Int. Society of Quantitative Pathology

Representative Publications:

189 scientific publications, among them 1 monography, 93 original articles (50 are indexed in PubMed).

Je. Erenpreisa. Lysis of cell nuclei on perforated electron microscopic supports. - Microsc.Acta, 1982, vol. 85, pp. 369-371.

Je. Erenpreisa et al. Apoptotic chromatin favours aggregation and fluorescence quenching of DNA dyes. - Histochem. & Cell Biol., 1997, vol.108, pp. 67-75.

Je.A. Erenpreisa. Organisation of the Chromatin in the Interphase Cell Nucleus, 1990, Riga: Zinatne, 115 pp. (in Russian)

Je. Erenpreisa et al. The chromatin network: Image analysis of differentiating chick embryo chondrocytes. - Eur.J.Histochem., 1993, vol. 37, pp.139-147.

Je. Erenpreisa et al. Influence of single-strand DNA breaks on ultrastructural and cytochemical organisation of the tumour cell chromatin. - Bull.Exp.Biol.Med., 1988, vol. 106, pp. 591-593 (in Russian, Engl. transl.).

T Arshavskaya, Je. Erenpreisa, H.Roach. Differentiation and programmed death in Sa-45 tumour treated with bone inducer. - Cell Biol.Internat., 1994, vol. 18, pp. 927-935.

H.I. Roach, Je. Erenpreisa, T. Aigner. Osteogenic differentiation of hypertrophic chondrocytes involves asymmetric cell divisions and apoptosis. - J.Cell Biol., 1995, vol. 131, pp. 483-494.

Je. Erenpreisa, H.I.Roach. Epigenetic selection as a possible component of transdifferentiation. - Mech.Ageing & Devel., 1996, vol.87, pp.165-182.

Je. Erenpreisa, H.I.Roach. Aberrant death in dark chondrocytes of the avian growth plate. - Cell Death & Differ, 1998, vol.5, pp.60-66.

Je. Erenpreisa et al. Arrest in metaphase and anatomy of mitotic catastrophe: mild heat shock in two human osteosarcoma cell lines. - Cell Biol. Internat., 2000, vol. 24, pp. 61-70.

T.Illidge, M.Cragg, B.Fringe, P.Olive, Je.Erenpreisa. Polyploid giant cells provide a survival mechanism for p53 mutant cells after DNA damage. - Cell Biol. Internat., 2000, vol. 24, pp. 635-648.

Je.Erenpreisa, M.Cragg, B.Fringe, I.Sharkhov, T.Illidge. Release of mitotic descendants by giont cells from irradiated Burkitt lymphoma cell lines. - Cell Biol. Internat., 2000, vol. 24, pp. ............

Je.Erenpreisa. " Tamed" chaos in embryonal development and carcinogenesis: a holistic view. - Proc. Latv. Acad. Sci, 2000, vol. 54, N 1/2, pp. 1-8.
J.Erenpreiss, J.Bars, V.Lipatnikova, Je. Erenpreisa and J. Zalkalns. Comparative Study of Cytochemical Tests for Sperm Chromatin Integrity. - Journal of Andrology, 2001, vol. 22, pp. 45-53.
J.Erenpreiss, S.Hlevicka, J. Zalkalns, Je. Erenpreisa. Effect of leukocytospermia on sperm DNA integrity: A negative effect in abnormal semen samples. - Journal of Andrology, 2002, vol. 23, pp. 717-723.
Je. Erenpreisa, A.Ivanov, M.Cragg, G.Selivanova, T.Illidge. Nuclear envelope-limited chromatin sheets are part of mitotic death. - Histochem. & Cell. Biol., 2002, vol. 117, pp. 243-255.
Je.Erenpreisa , J.Erenpreiss, T. Freivalds, M.Slaidina , R.Krampe, J.Butikova , A.Ivanov , D. Pjanova. Toluidine blue test for sperm DNA integrity and elaboration of image cytometry algorithm.- Cytometry, 2003, vol. 52A, pp. 19-27.
A.Ivanov, M.Cragg, Je.Erenpreisa, D.Emzinsh, H.Lukman, T.Illidge. Endopolyploid tumour cells produced after severe genotoxic damage have the potential to repair DNA double strand breaks. - J.Cell Sci. 2003, 116:4095- 4106.
J. Erenpreiss, K.Jepson, A.Giwercman, I.Tsarev, Je. Erenpreisa, M .Spano. Toluidine blue cytomerty test for sperm DNA conformation: comparison with the flow cytometric sperm chromatin structure and TUNEL assays. - Hum. Reprod., 2004, vol. 19, pp. 2277-2282.
Erenpreisa J, Kalejs M, Ianzini F, Kosmacek EA, Mackey MA, Emzinsh D, Cragg MS, Ivanov A, Illidge TM. Segregation of genomes in polyploid tumour cells following mitotic catastrophe. - Cell Biol Int, 2005, 29: 1005-1011
Kalejs M, Ivanov A, Plakhins G, Cragg MS, Emzins Dz , Illidge TM, Erenpreisa Je. Upregulation of meiosis-specific genes in lymphoma cell lines following genotoxic insult and the induction of mitotic catastrophe. BMC Cancer, 2006, doi: 10.1186/1471-2407-6-6.
Erenpreiss J, Spano M, Erenpreisa J, Bungum M, Giwercman A. Sperm chromatin structure and male fertility: biological and clinical aspects. - Asian J. Androl., 2006, 8:11-29.
J.Erenpreisa, M.S.Cragg. Cancer: a matter of life-cycle?. - Cell Biol., 2007, vol. 31, pp. 1507-1510.

Participation in International Conferences:

45 European and International Congresses and Conferences, 1989-2006.

Present International collaboration -

England:
           - Prof. Dr. T. Illidge (Paterson Cancer Research Institute, Manchester) - radiotherapy of resistant lymphomas.
           - Dr. Mark Cragg (Wessex Cancer Division) - resistant to therapy lymphomas, molecular methods.
Germany
- Prof. Dr. M. Hausmann (Kirchhoff Inst. of Physics, Heidelberg) – Cell nucleus structural FISH analysis
Sweden
- Prof. A. Giwercman (Scanian Andrology Centre, Lund University) – sperm chromatin structure in infertility analysis
USA
- Dr. M. MacKey and Dr. F. Ianzini – (Dept Biomedical Engineer. and Dept. Radiat. Oncol. Iowa University) – live imaging of tumour cells
Japan
- Assoc. Prof. Dr. S.Matsumoto (Fac. Education and Human Sci, Yokohama National University) – physical chemistry of structural dyes.

Recent Research Projects:

Local (supported by the Latvian Council of Science ):

Je. Erenpreisa (Head of the Project). Significance of the Genome Oscillations in the Programmed Cell Death (1994-1996).

Je. Erenpreisa (Head of the Project, 1997 -) . Relationships Between Proliferation and Apoptosis in Experimental Tumours After Genotoxic Insult. (Within State Program "Malignant Tumours", 1996-1999).

Je. Erenpreisa (Head of the Project). Sensitive Test for DNA Strand-Breaks: Its Elaboration and Application to Detect the Early Apoptosis of Somatic and the Nuclear Status of Sperm Cells (1997-1999).

Je. Erenpreisa (Head of the Project). The Role of MOS/MAPK Pathway in the Genotoxic Resistance of P53 Mutat Lymphoblastoid Tumors. (2001 - ).

Je. Erenpreisa (Head of the Project). Natural Genotoxic Resistance of Malignant Tumors: Mechanisms and Prognosis (2001 -2004 ).

Je. Erenpreisa. Signalling, Repair of DNA Double-Strand Breaks and Their Relationship with Apoptosis in Lymphoblastoid Cell Lines, with Different Resistance to Genotoxic Damage (2005 - ).

New skrinning instruments of anti-tumour drugs (2006 - ) – State programme

English-Latvian FSU joint projects (supported by the Royal Society of London):

Je. Erenpreisa (Head of the Latvian Part of Project). Transdifferentiation of Hypertrophic Chondrocytes Into Bone-Forming Cells (1993-1996).

Je. Erenpreisa (Head of the Latvian Part of Project). The Role of Some Oncogenes in Genome Reprogramming During Transdifferentiation (1997-1999).

Je. Erenpreisa (Head of the Latvian Part of Project). The Role of Giant Cells in Genotoxic Resistance of Lymphomas (2000-2002).

Bilateral Germany-Latvia BMBF project (2006-2007): “Spatial organisation of genomes and homologous chromosomes in endopolyploid tumour cells”

USA-Latvian visits exchange project (2005):

Determination of clonogenic survival capacity and its origin in tumor cells undergoing mitotic catastrophe: implication for radiation treatment (head of the Latvian part)..

Last update 29.02.2008